Data Analysis

The iubfccf has two options for data analysis - FCS Express and FlowJo. FCS Express and FlowJo are both powerful off-line cytometry data analysis software programs. Both programs can analyze all flow cytometry data files in FCS format, and are compatible with both Mac and Windows systems.

FCS Express

The facility has an internet site license for the FCS Express program. Any IU researcher may download the program and run it on their computer via an internet connection - no dongle is necessary. The cost per license is $260/registration annually (starting July 1, 2025) and includes unlimited use of FCS Express. The license registration will renew yearly on July 1 with a valid IU lab account number. Please note that registration is not prorated.

Interested in testing FCS Express?

First register for a De Novo account at: https://denovosoftware.com/register/ and then go here: https://denovosoftware.com/demo-overview/

Interested in purchasing an FCS Express license seat?

If you are interested in purchasing a license, submit a request at: https://www.denovosoftware.com/RegisterForSiteLicense/?SignupCode=IUBFCCF - and email the facility with a valid account number.

Annual FCS Express training

The site license comes with yearly training which will be announced via the flow facility listserv. Webinars can be found at: webinars (link).

FlowJo

The facility currently has two (2) copies of the program available in dongle (USB) format that may be signed out. A copy of the FlowJo program can be downloaded at https://www.flowjo.com/flowjo/download. Both traveling dongles can be used on Apple and Windows systems. Please note that dongles are not compatible with FlowJo version 11.

FlowJo tutorials and videos

FlowJo tutorials and videos can be found on the links page of the website.

FlowJo Reservations

Reservations for the FlowJo dongle must be made at least one full business day in advance. The maximum sign-out time for the traveling dongle is: 24 hours (or through the weekend if requested on a Friday).
Extensions may be provided on a case-by-case basis.
Please contact core staff to schedule an appointment for use of a FlowJo dongle and for more information.

Important note about FlowJo dongles

If a dongle is damaged or lost, it can no longer be replaced. FlowJo is no longer selling dongles.

Data Analysis

FCS Express

Interested in testing FCS Express?

First register for a De Novo account at: https://denovosoftware.com/register/ and then go here: https://denovosoftware.com/demo-overview/

Interested in purchasing an FCS Express license seat?

If you are interested in purchasing a license, submit a request at: https://www.denovosoftware.com/RegisterForSiteLicense/?SignupCode=IUBFCCF - and email the facility with a valid account number.

Annual FCS Express training

The site license comes with yearly training which will be announced via the flow facility listserv. Webinars can be found at: webinars (link).

FlowJo

FlowJo tutorials and videos

FlowJo tutorials and videos can be found on the links page of the website.

FlowJo Reservations

Reservations for the FlowJo dongle must be made at least one full business day in advance. The maximum sign-out time for the traveling dongle is: 24 hours (or through the weekend if requested on a Friday).

Extensions may be provided on a case-by-case basis.

Please contact core staff to schedule an appointment for use of a FlowJo dongle and for more information.

Important note about FlowJo dongles

If a dongle is damaged or lost, it can no longer be replaced. FlowJo is no longer selling dongles.