Forms and Protocols
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The following PDF contains useful information about the facility and sample preparation:
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FCCF info (click on PDF icon)
Please fill out and submit the following forms when submitting a new project or using the Aria IIu or SH800:
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Project description form (click on PDF icon)
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IBC/Biohazard form - if sample has an IBC protocol and/or is a biohazard (click on PDF icon)
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Aria II analysis or sorting form (click on PDF icon)
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SH800 form (link)​
These protocols have been used by researchers at IUB:
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Apoptosis detection – Annexin V-AF488 vs PI (Thermo Fisher)
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Cell Cycle – Propidium Iodide (UCSD) - add ethanol to cells
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Cell Proliferation (Thermo Fisher)
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Drosophila Embyro sorting (pdf)
Useful guides:
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Sorting Guidelines (CCHMC RFCC)
Additional assays and sorting performed by researchers at IU Bloomington:
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Surface marker Staining and sorting (Stem & Adherent cells - BD Biosciences)
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Intracellular marker staining (BioLegend)
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Measurement of Green Fluorescent Protein (GFP) expression and sorting
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Single Cell Sorting
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Mitochondrial membrane potential (ThermoFisher)
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Viability assays (Live/Dead dyes - BioLegend)
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Bacterial viability & counting (Viability; Viability & counting - Thermo Fisher)
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Protocol for Sulfolobus fixation and cell cycle analysis available through IUCAT - Analysis of the Archaeal ESCRT Apparatus
Other assays available:
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BrdU incorporation
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Cell proliferation assays
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Intracellular Cytokine detection
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Multiparameter Immunofluorescence analysis and cell sorting
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Signal Transduction
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and much more!
Other resources for cell preparation and staining for flow cytometry
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Current Protocols in Cytometry – (Facility has a copy on permanent loan from Libraries)
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COPAS Select, Union Biometrica - search protocols
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Flow Cytometry with Plant Cells (Wiley, E-Book, IU-B Life Sciences Library)